Overview

  • Product name
  • Description
    Mouse monoclonal to KIAA1199
  • Tested applications
    Suitable for: ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Recombinant tagged fragment: LYDGPINIQN CTFRKFVALE GRHTSALAFR LNNAWQSCPH NNVTGIAFED VPITSRVFFG EPGPWFNQLD MDGDKTSVFH DVDGSVSEYP GSYLTKNDNW , corresponding to amino acids 880-980 of Human KIAA1199

Properties

Applications

Our Abpromise guarantee covers the use of ab76849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 153 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      KIAA1199 belongs to the TMEM2 family and contains 4 PbH1 repeats. It is expressed in the inner ear. It is expressed in cochlea and vestibule tissues and it may be involved in hearing. Defects in KIAA1199 may be a cause of non-syndromic hearing loss. It is also thought that KIAA1199 is a tumor suppressor that may be involved in establishing cellular mortality in normal human cells.
    • Database links
    • Alternative names
      • IR2155535 antibody
      • KIAA1199 protein antibody
      • OTTHUMP00000185569 antibody
      • OTTHUMP00000195036 antibody
      • TMEM2L antibody
      • Transmembrane protein 2 like antibody
      see all

    Anti-KIAA1199 antibody images

    • Anti-KIAA1199 antibody (ab76849) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 153 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    References for Anti-KIAA1199 antibody (ab76849)

    This product has been referenced in:
    • Jia S  et al. KIAA1199 promotes migration and invasion by Wnt/ß-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer. PLoS One 12:e0175058 (2017). IHC-P ; Human . Read more (PubMed: 28422983) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human recombinant protein of KIAA1199 in COS-1 cel)
    Specification
    human recombinant protein of KIAA1199 in COS-1 cel
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100 in PBS
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Mr. Cem Kuscu

    Verified customer

    Submitted Dec 29 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (COS-1 with recombinant KIAA1199,and cell lysates f)
    Loading amount
    30 µg
    Specification
    COS-1 with recombinant KIAA1199,and cell lysates f
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Mr. Cem Kuscu

    Verified customer

    Submitted Dec 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up