Overview

  • Product nameAnti-KIAA1462 antibody
    See all KIAA1462 primary antibodies
  • Description
    Rabbit polyclonal to KIAA1462
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 1053-1186 of Human KIAA1462.

  • Positive control
    • Human rectum tissue; RT4 and U 251 MG cell lysate; Human liver and tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-KIAA1462 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane. Recommended concentration of ab121545 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121545 at 1/15 dilution staining KIAA1462 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-KIAA1462 antibody (ab121545) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-KIAA1462 antibody (ab121545)

ab121545 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"