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Rabbit polyclonal to KIAA1462
antigen sequence, corresponding to internal sequence amino acids 1053-1186 of Human KIAA1462.
- Human rectum tissue; RT4 and U 251 MG cell lysate; Human liver and tonsil lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- JCAD antibody
- Junctional protein associated with coronary artery disease antibody
- K1462_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti- KIAA1462 antibody (ab121545)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane. Recommended concentration of ab121545 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- KIAA1462 antibody (ab121545)
ab121545 at 1/15 dilution staining KIAA1462 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
Western blot - Anti- KIAA1462 antibody (ab121545)
All lanes : Anti-KIAA1462 antibody (ab121545) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"