The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMicrotubule minus-end binding protein that acts as a regulator of microtubule dynamics. Specifically required for zonula adherens biogenesis and maintenance by anchoring microtubules at their minus-ends to zonula adherens, leading to recruit KIFC3 kinesin to junctional site.
Sequence similaritiesBelongs to the CAMSAP1 family. Contains 1 CH (calponin-homology) domain. Contains 1 CKK domain.
DomainThe CKK domain binds microtubules.
Cellular localizationCell junction > adherens junction. Cytoplasm. Cytoplasm > cytoskeleton. Scattered in the cytoplasm. Localizes along zonula adherens only at mature cell-cell contacts.