The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Involvement in disease
Note=A chromosomal aberration involving KIAA1549 is found in pilocytic astrocytoma. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
Predicted band size : 90 kDa Western blot analysis of Mouse neurosphere lysate (30µg/lane) labeling KIAA159 with ab118721 at 1:1000. A HRP-conjugated anti-rabbit (1:5000) was used as the secondary antibody.
Western blot - Anti- KIAA1549 antibody (ab118721)
Anti-KIAA1549 antibody (ab118721) at 1 µg/ml + HepG2 cell lysate at 10 µg