Overview

  • Product nameAnti-KIAA1576 antibody
    See all KIAA1576 primary antibodies
  • Description
    Mouse polyclonal to KIAA1576
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human KIAA1576 protein (NP_065978).

  • Positive control
    • KIAA1576 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Sequence similaritiesBelongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Probable oxidoreductase KIAA1576 antibody
    • Synaptic vesicle membrane protein VAT 1 homolog like antibody
    • Synaptic vesicle membrane protein VAT-1 homolog-like antibody
    • VAT 1L antibody
    • VAT1L antibody
    • VAT1L_HUMAN antibody
    • Vesicle amine transport protein 1 homolog (T. californica) like antibody
    see all

Anti-KIAA1576 antibody images

  • All lanes : Anti-KIAA1576 antibody (ab68926) at 1/500 dilution

    Lane 1 : KIAA1576 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab68926 staining KIAA1576 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 4°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

References for Anti-KIAA1576 antibody (ab68926)

ab68926 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"