Overview

  • Product nameAnti-KIF12 antibody
    See all KIF12 primary antibodies
  • Description
    Rabbit polyclonal to KIF12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: LLSLGSPRPL PVRWNKTRGF YVEQLRVVEF GSLEALMELL QTGLSRRRNS , corresponding to N terminal amino acids 36 - 85 of Human KIF12 (NP_612433)

  • Positive control
    • 721 B cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab80448 is purified by a peptide affinity chromatography method
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

Anti-KIF12 antibody images

  • Anti-KIF12 antibody (ab80448) at 1 µg/ml + 721 B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 - 1/100000

    Predicted band size : 71 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

References for Anti-KIF12 antibody (ab80448)

This product has been referenced in:
  • Tan MH  et al. Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer. Breast Cancer Res Treat 131:849-58 (2012). WB ; Human . Read more (PubMed: 21479552) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"