Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF14
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.

  • Positive control
    • HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/250 - 1/1000.

Target

  • Function
    Plays an essential role in cytokinesis.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • Developmental stage
    Up-regulated in cells progressing through G2/M phase.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 190 kDa

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

  • Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lane 1 - Input.

    Lane 2 - IP.

    Lane 3 - Control IgG IP.

References

ab3747 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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