The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P (formaldehyde fixed): 1/100 - 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Plays an essential role in cytokinesis.
Belongs to the kinesin-like protein family. Contains 1 FHA domain. Contains 1 kinesin-motor domain.
Up-regulated in cells progressing through G2/M phase.
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.