Anti-KIF15 antibody (ab90735)

Overview

  • Product nameAnti-KIF15 antibody
    See all KIF15 primary antibodies
  • Description
    Rabbit polyclonal to KIF15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A synthetic peptide corresponding to a region within internal sequence amino acids 1080 - 1129 (

    KKHSGLLQSAQEELTKKEALIQELQHKLNQKKEEVEQKKNEYNFKMRQLE

    ) of Human KIF15 (NP_064627)

  • Positive control
    • COLO205 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceKIF15 belongs to the kinesin-like protein family and contains 1 kinesin-motor domain. KIF15 is the plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.
  • Cellular localizationCytoplasmic, Cytoskeleton, Microtubule Note: Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase.
  • Database links
  • Alternative names
    • FLJ25667 antibody
    • Kinesin like protein 7 antibody
    • KLP2 antibody
    • HKLP2 antibody
    • Kinesin family member 15 antibody
    • Kinesin like protein 2 antibody
    • Kinesin like protein KIF15 antibody
    • KNSL7 antibody
    • Linesin like 7 antibody
    • NY BR 62 antibody
    • NYBR62 antibody
    • Serologically defined breast cancer antigen NY BR 62 antibody
    • Serologically defined breast cancer antigen NYBR62 antibody
    see all

Anti-KIF15 antibody images

  • Anti-KIF15 antibody (ab90735) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 160 kDa

References for Anti-KIF15 antibody (ab90735)

ab90735 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 27 2015

Thanks for your reply.

I received a reply from my lab colleagues who informed that the intense band at 65-70kD is either a degradation product of the full-length intact protein, or a non-specific band altogether. We are not absolutely sure ex...

Read More

Thank you for your reply.

Although we don’t normally offer discount codes for two different untested species / applications, I am happy to make an exception in this occasion as the antibody shares high homology with the mouse protein. <...

Read More

Thank you for contacting us.

Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab90735.

Unfortunately ab90735 has not be...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"