The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionMicrotubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.
Sequence similaritiesBelongs to the kinesin-like protein family. Contains 1 kinesin-motor domain.
Detection of KIF18A by Western Blot of Immunprecipitate.
anti-KIF18A at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72416 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-KIF18A antibody (ab72416)
This product has been referenced in:
Chen QI et al. Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma. Exp Ther Med12:377-383 (2016).
Read more (PubMed: 27347065) »