Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF18A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 848 and the C-terminus (residue 898) of human KIF18A (NP_112494.2)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab72417 was affinity purified using an epitope specific to KIF18A immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Glycosylated.
    Ubiquitinated.
  • Cellular localization
    Cell projection > ruffle. Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • AU024633 antibody
    • B130001M12Rik antibody
    • DKFZp434G2226 antibody
    • KI18A_HUMAN antibody
    • KIF 18A antibody
    • KIF18A antibody
    • kinesin family member 18A antibody
    • Kinesin like protein KIF18A antibody
    • Kinesin-like protein KIF18A antibody
    • Marrow stromal KIF18A antibody
    • MGC27627 antibody
    • MS KIF18A antibody
    • MS-KIF18A antibody
    • RGD1561740 antibody
    • RP23 206C1.1 antibody
    see all

Images

  • All lanes : Anti-KIF18A antibody (ab72417) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of KIF18A by Western Blot of Immunprecipitate.
    ab72417 at 1µg/ml staining KIF18A in HeLa whole cell lysate immunoprecipitated using ab72417 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab72417 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab72417.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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