Overview

  • Product nameAnti-KIF1B antibody
    See all KIF1B primary antibodies
  • Description
    Rabbit polyclonal to KIF1B
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human KIF1B.

    (Peptide available as ab74827.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysate: Mouse Brain, 0 days old.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab69614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 204 kDa).

Target

  • RelevanceKIF1B, or kinesin-like protein (Klp) functions as a motor for mitochondrial transport, and has a microtubule plus end-directed motility. The KIF1B beta isoform is abundant in brain, while the alpha isoform is abundant in skeletal muscle. Mutations in the KIF1B gene are the cause of Charcot-Marie-Tooth disease type 2A1, which is a primary peripheral axon neuropathy. The KIF1B beta isoform is down-regulated in sporadic amyotrophic lateral sclerosis (ALS).
  • Cellular localizationCytoplasmic and Mitochondrial
  • Database links
  • Alternative names
    • Charcot Marie Tooth neuropathy 2A antibody
    • CMT 2 antibody
    • CMT 2A antibody
    • CMT 2A1 antibody
    • CMT2 A antibody
    • CMT2 A1 antibody
    • CMT2 antibody
    • CMT2A 1 antibody
    • CMT2A antibody
    • CMT2A1 antibody
    • D4Mil1e antibody
    • HMSN II antibody
    • HMSNII antibody
    • HMSNII, hereditary motor sensory neuropathy II antibody
    • KIF 1B antibody
    • KIF1 B antibody
    • KIF1B p130 antibody
    • KIF1B p204 antibody
    • KIF1Bp130 antibody
    • KIF1Bp204 antibody
    • kinesin family member 1B antibody
    • Kinesin like protein KIF1B antibody
    • Klp antibody
    see all

Anti-KIF1B antibody images

  • Anti-KIF1B antibody (ab69614) at 2 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 204 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 230 kDa,45 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab69614 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69614, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-KIF1B antibody (ab69614)

ab69614 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (DRG)
Specification DRG
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"