Overview

  • Product nameAnti-KIF1C antibody
    See all KIF1C primary antibodies
  • Description
    Rabbit polyclonal to KIF1C
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 850 and 900 of human KIF1C (NP_006603.2)

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 123 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMotor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility.
  • Tissue specificityExpressed in all tissues examined, with most abundant expression in heart and skeletal muscle.
  • Involvement in diseaseSpastic ataxia 2, autosomal recessive
  • Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily.
    Contains 1 FHA domain.
    Contains 1 kinesin motor domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kif1c antibody
    • KIF1C_HUMAN antibody
    • Kinesin family member 1C antibody
    • Kinesin-like protein KIF1C antibody
    • LTXS1 antibody
    see all

Anti-KIF1C antibody images

  • All lanes : Anti-KIF1C antibody (ab72238) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg


    Predicted band size : 123 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation/ Western Blot of KIF1C.
    Lane 1: ab72238 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72238 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.
  • IHC image of ab72238 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72238, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-KIF1C antibody (ab72238)

This product has been referenced in:
  • Jones LA  et al. Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion. J Cell Biol 207:499-516 (2014). Read more (PubMed: 25422374) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"