Overview

  • Product nameAnti-KIF20A antibody
    See all KIF20A primary antibodies
  • Description
    Rabbit polyclonal to KIF20A
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 73-229 of Human KIF20A (AAH12999)

  • Positive control
    • HepG2 cell lysate, Mouse brain tissue lysate, H441 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab104118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 100 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
  • Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA415432 antibody
    • FLJ21151 antibody
    • GG10 2 antibody
    • GG10_2 antibody
    • KI20A_HUMAN antibody
    • KIF20A antibody
    • kinesin family member 20A antibody
    • Kinesin like protein KIF20A antibody
    • Kinesin-like protein 174 antibody
    • Kinesin-like protein KIF20A antibody
    • mitotic kinesin like protein 2 antibody
    • Mitotic kinesin-like protein 2 antibody
    • MKlp2 antibody
    • OTTHUMP00000159481 antibody
    • RAB6 interacting, kinesin like (rabkinesin6) antibody
    • Rab6-interacting kinesin-like protein antibody
    • RAB6-INTERACTING PROTEIN, KINESIN-LIKE antibody
    • RAB6KIFL antibody
    • Rabkinesin 6 antibody
    • Rabkinesin-6 antibody
    see all

Anti-KIF20A antibody images

  • Anti-KIF20A antibody (ab104118) at 1/2000 dilution + HepG2 cell lysate at 30 µg

    Predicted band size : 100 kDa
  • Anti-KIF20A antibody (ab104118) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Predicted band size : 100 kDa
  • Immunohistochemical analysis of paraffin-embedded H441 xenograft using ab104118 at 1/250 dilution.

References for Anti-KIF20A antibody (ab104118)

ab104118 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"