Overview

  • Product nameAnti-KIF20A antibody
    See all KIF20A primary antibodies
  • Description
    Rabbit polyclonal to KIF20A
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence

    KRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRR

    , amino acids 828-877 of Human KIF20A (NP_005724)

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab85644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
  • Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA415432 antibody
    • FLJ21151 antibody
    • GG10 2 antibody
    • GG10_2 antibody
    • KI20A_HUMAN antibody
    • KIF20A antibody
    • kinesin family member 20A antibody
    • Kinesin like protein KIF20A antibody
    • Kinesin-like protein 174 antibody
    • Kinesin-like protein KIF20A antibody
    • mitotic kinesin like protein 2 antibody
    • Mitotic kinesin-like protein 2 antibody
    • MKlp2 antibody
    • OTTHUMP00000159481 antibody
    • RAB6 interacting, kinesin like (rabkinesin6) antibody
    • Rab6-interacting kinesin-like protein antibody
    • RAB6-INTERACTING PROTEIN, KINESIN-LIKE antibody
    • RAB6KIFL antibody
    • Rabkinesin 6 antibody
    • Rabkinesin-6 antibody
    see all

Anti-KIF20A antibody images

  • Anti-KIF20A antibody (ab85644) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 100 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab85644 staining KIF20A in spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab85644 staining KIF20A in thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-KIF20A antibody (ab85644)

ab85644 has not yet been referenced specifically in any publications.

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