Anti-KIF20A (phospho S528) antibody (ab63547)

Overview

  • Product nameAnti-KIF20A (phospho S528) antibody
    See all KIF20A primary antibodies
  • Description
    Rabbit polyclonal to KIF20A (phospho S528)
  • SpecificityDetects endogenous levels of KIF20A only when phosphorylated at serine 528.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human KIF20A around the phosphorylation site of serine 528 (E-H-SP-L-Q).

  • Positive control
    • Extracts from 293 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
ELISA 1/5000.

Target

  • FunctionMitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
  • Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA415432 antibody
    • FLJ21151 antibody
    • GG10 2 antibody
    • GG10_2 antibody
    • KI20A_HUMAN antibody
    • KIF20A antibody
    • kinesin family member 20A antibody
    • Kinesin like protein KIF20A antibody
    • Kinesin-like protein 174 antibody
    • Kinesin-like protein KIF20A antibody
    • mitotic kinesin like protein 2 antibody
    • Mitotic kinesin-like protein 2 antibody
    • MKlp2 antibody
    • OTTHUMP00000159481 antibody
    • RAB6 interacting, kinesin like (rabkinesin6) antibody
    • Rab6-interacting kinesin-like protein antibody
    • RAB6-INTERACTING PROTEIN, KINESIN-LIKE antibody
    • RAB6KIFL antibody
    • Rabkinesin 6 antibody
    • Rabkinesin-6 antibody
    see all

Anti-KIF20A (phospho S528) antibody images

  • All lanes : Anti-KIF20A (phospho S528) antibody (ab63547) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from 293 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 100 kDa
    Observed band size : 100 kDa

References for Anti-KIF20A (phospho S528) antibody (ab63547)

ab63547 has not yet been referenced specifically in any publications.

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