- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-KIF20B antibody
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DescriptionRabbit polyclonal to KIF20B
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
antigen sequence, corresponding to amino acids 663-811 of Human KIF20B, isoform 3.
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Positive control
- Human duodenum tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab122165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionPlus-end-directed motor enzyme that is required for completion of cytokinesis.
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Tissue specificityBrain, ovary, kidney and testis.
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Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules. A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase. During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone. At anaphase it is concentrated in the mid-body.
- Information by UniProt
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Database links
- Entrez Gene: 9585 Human
- Omim: 605498 Human
- SwissProt: Q96Q89 Human
- Unigene: 240 Human
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Alternative names
- Cancer/testis antigen 90 antibody
- CT90 antibody
- KI20B_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KIF20B antibody (ab122165)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122165 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF20B antibody (ab122165)ab122165 at 1/75 dilution staining KIF20B in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.
References
ab122165 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"