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Overview

  • Product nameAnti-KIF20B antibody
  • Description
    Rabbit polyclonal to KIF20B
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 663-811 of Human KIF20B, isoform 3.

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPlus-end-directed motor enzyme that is required for completion of cytokinesis.
  • Tissue specificityBrain, ovary, kidney and testis.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules. A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase. During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone. At anaphase it is concentrated in the mid-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • Cancer/testis antigen 90 antibody
      • CT90 antibody
      • KI20B_HUMAN antibody
      • KIF20B antibody
      • Kinesin-like protein KIF20B antibody
      • Kinesin-related motor interacting with PIN1 antibody
      • KRMP1 antibody
      • M-phase phosphoprotein 1 antibody
      • MPHOSPH1 antibody
      • MPP 1 antibody
      • Mpp1 antibody
      see all

    Anti-KIF20B antibody images

    • Immunocytochemistry/ Immunofluorescence - Anti-KIF20B antibody (ab122165)
      Immunocytochemistry/ Immunofluorescence - Anti-KIF20B antibody (ab122165)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122165 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF20B antibody (ab122165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF20B antibody (ab122165)
      ab122165 at 1/75 dilution staining KIF20B in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.

    References for Anti-KIF20B antibody (ab122165)

    ab122165 has not yet been referenced specifically in any publications.

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