Customer reviews and FAQs

Overview

  • Product name
    Anti-KIF20B antibody
  • Description
    Rabbit polyclonal to KIF20B
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 663-811 of Human KIF20B, isoform 3.

  • Positive control
    • Human duodenum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plus-end-directed motor enzyme that is required for completion of cytokinesis.
  • Tissue specificity
    Brain, ovary, kidney and testis.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules. A 2-3 fold expression increase is seen as cells progress from G1 to G2/M phase. During prophase and metaphase it is found throughout the cytoplasm and at anaphase accumulates at the midplan of the cell and forms a distinct band extending across the spindle midzone. At anaphase it is concentrated in the mid-body.
  • Information by UniProt
  • Database links
    • Alternative names
      • Cancer/testis antigen 90 antibody
      • CT90 antibody
      • KI20B_HUMAN antibody
      • KIF20B antibody
      • Kinesin-like protein KIF20B antibody
      • Kinesin-related motor interacting with PIN1 antibody
      • KRMP1 antibody
      • M-phase phosphoprotein 1 antibody
      • MPHOSPH1 antibody
      • MPP 1 antibody
      • Mpp1 antibody
      see all

    Anti-KIF20B antibody images

    • Immunocytochemistry/ Immunofluorescence - Anti-KIF20B antibody (ab122165)
      Immunocytochemistry/ Immunofluorescence - Anti-KIF20B antibody (ab122165)
      Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122165 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF20B antibody (ab122165)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF20B antibody (ab122165)
      ab122165 at 1/75 dilution staining KIF20B in Paraffin-embedded Human duodenum tissue by Immunohistochemistry.

    References for Anti-KIF20B antibody (ab122165)

    ab122165 has not yet been referenced specifically in any publications.

    Publishing research using ab122165? Please let us know so that we can cite the reference in this datasheet.

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