Anti-KIF22 antibody (ab56235)

Overview

  • Product nameAnti-KIF22 antibody
    See all KIF22 primary antibodies
  • Description
    Rabbit polyclonal to KIF22
  • Tested applicationsSuitable for: WB, ELISAmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MVLMKTVEEK DLEIERLKTK QKELEAKMLA QKAEEKENHC PTMLRPLSHR, corresponding to C terminal amino acids 469-518 of Human KIF22

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab56235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 73 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionKinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
    • Sequence similaritiesBelongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      see all

    Anti-KIF22 antibody images

    • Anti-KIF22 antibody (ab56235) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 73 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 64 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-KIF22 antibody (ab56235)

    ab56235 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"