Anti-KIF22 antibody [EP2747Y] (ab75783)

Overview

  • Product nameAnti-KIF22 antibody [EP2747Y]
    See all KIF22 primary antibodies
  • Description
    Rabbit monoclonal [EP2747Y] to KIF22
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on the N-terminus of KIF22

  • Positive control
    • HeLa cell lysate
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP 1/10.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionKinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
  • Sequence similaritiesBelongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Post-translational
    modifications
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • A 328A3.2 antibody
    • KID antibody
    • KIF 22 antibody
    • kif22 antibody
    • KIF22_HUMAN antibody
    • Kinesin family member 22 antibody
    • Kinesin like 4 antibody
    • Kinesin like DNA binding protein antibody
    • Kinesin like DNA binding protein pseudogene antibody
    • Kinesin like protein 4 antibody
    • Kinesin like protein KIF22 antibody
    • Kinesin-like DNA-binding protein antibody
    • Kinesin-like protein 4 antibody
    • Kinesin-like protein KIF22 antibody
    • KNSL 4 antibody
    • KNSL4 antibody
    • OBP 1 antibody
    • OBP 2 antibody
    • OBP antibody
    • OBP1 antibody
    • OBP2 antibody
    • Origin of plasmid DNA replication binding protein antibody
    • OriP binding protein antibody
    • OTTHUMP00000123406 antibody
    see all

Anti-KIF22 antibody [EP2747Y] images

  • Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled oat anti rabbit at 1/2000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-KIF22 antibody [EP2747Y] (ab75783)

ab75783 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - nuclear (cartilage)
Loading amount 10 µg
Specification cartilage
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Oct 26 2012

Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"