Anti-KIF22 (phospho S427) antibody [EPR2728Y] (ab76182)

Overview

  • Product nameAnti-KIF22 (phospho S427) antibody [EPR2728Y]
    See all KIF22 primary antibodies
  • Description
    Rabbit monoclonal [EPR2728Y] to KIF22 (phospho S427)
  • Specificityab76182 only detects KIF22 phosphorylated on Serine 427.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide from residues surrounding Serine 427 of human KIFF22.

  • Positive control
    • HeLa cell lysate treated with aphidicolin + nocodazole.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 73 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionKinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
    • Sequence similaritiesBelongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      see all

    Anti-KIF22 (phospho S427) antibody [EPR2728Y] images

    • All lanes : Anti-KIF22 (phospho S427) antibody [EPR2728Y] (ab76182) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with aphidicolin + nocodazole

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit-HRP at 1/2000 dilution

      Predicted band size : 73 kDa
      Observed band size : 73 kDa

    References for Anti-KIF22 (phospho S427) antibody [EPR2728Y] (ab76182)

    ab76182 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"