Recombinant
RabMAb

Anti-KIF22 (phospho S427) antibody [EPR2728Y] (ab76182)

Overview

  • Product name
    Anti-KIF22 (phospho S427) antibody [EPR2728Y]
    See all KIF22 primary antibodies
  • Description
    Rabbit monoclonal [EPR2728Y] to KIF22 (phospho S427)
  • Specificity
    ab76182 only detects KIF22 phosphorylated on Serine 427.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide from residues surrounding Serine 427 of human KIFF22.

  • Positive control
    • HeLa cell lysate treated with aphidicolin + nocodazole.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 73 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
    • Sequence similarities
      Belongs to the kinesin-like protein family.
      Contains 1 kinesin-motor domain.
    • Post-translational
      modifications
      Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • A 328A3.2 antibody
      • KID antibody
      • KIF 22 antibody
      • kif22 antibody
      • KIF22_HUMAN antibody
      • Kinesin family member 22 antibody
      • Kinesin like 4 antibody
      • Kinesin like DNA binding protein antibody
      • Kinesin like DNA binding protein pseudogene antibody
      • Kinesin like protein 4 antibody
      • Kinesin like protein KIF22 antibody
      • Kinesin-like DNA-binding protein antibody
      • Kinesin-like protein 4 antibody
      • Kinesin-like protein KIF22 antibody
      • KNSL 4 antibody
      • KNSL4 antibody
      • OBP 1 antibody
      • OBP 2 antibody
      • OBP antibody
      • OBP1 antibody
      • OBP2 antibody
      • Origin of plasmid DNA replication binding protein antibody
      • OriP binding protein antibody
      • OTTHUMP00000123406 antibody
      • SEMDJL2 antibody
      see all

    Images

    • All lanes : Anti-KIF22 (phospho S427) antibody [EPR2728Y] (ab76182) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with aphidicolin + nocodazole

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit-HRP at 1/2000 dilution

      Predicted band size : 73 kDa
      Observed band size : 73 kDa

    References

    ab76182 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76182.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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