Overview

  • Product nameAnti-Kif2a antibody
    See all Kif2a primary antibodies
  • Description
    Rabbit polyclonal to Kif2a
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: all other Vertebrates
  • Immunogen

    N-terminal recombinant fragment (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab37005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 110 kDa.
IP 1/10000.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionPlus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
  • Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDCBM3 antibody
    • HK2 antibody
    • KIF2 antibody
    • KIF2A antibody
    • KIF2A_HUMAN antibody
    • Kinesin 2 antibody
    • Kinesin heavy chain 2 antibody
    • Kinesin heavy chain member 2 antibody
    • Kinesin heavy chain member 2A antibody
    • Kinesin like protein KIF2A antibody
    • Kinesin-2 antibody
    • Kinesin-like protein KIF2A antibody
    • KNS2 antibody
    • M Kinesin antibody
    see all

Anti-Kif2a antibody images

  • ab37005, at a 1/5000 dilution, staining HeLa cells fixed in 3.5% paraformaldehyde, by immunofluorescence. Centrosome staining during interphase and spindle pole staining during mitosis.
  • ab37005 at a 1/10000 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. A FITC conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References for Anti-Kif2a antibody (ab37005)

This product has been referenced in:
  • Wang J  et al. KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer. BMC Cancer 14:461 (2014). WB, IHC-P ; Human . Read more (PubMed: 24950762) »
  • Zaganjor E  et al. Ras transformation uncouples the kinesin-coordinated cellular nutrient response. Proc Natl Acad Sci U S A 111:10568-73 (2014). Human . Read more (PubMed: 25002494) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 15 µg
Specification HeLa
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Nov 30 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 12 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"