Overview

  • Product nameAnti-Kif2a antibody
    See all Kif2a primary antibodies
  • Description
    Mouse monoclonal to Kif2a
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-680 of Human Kif2a

Properties

Applications

Our Abpromise guarantee covers the use of ab55383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 6 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
    • Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
      Contains 1 kinesin-motor domain.
    • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDCBM3 antibody
      • HK2 antibody
      • KIF2 antibody
      • KIF2A antibody
      • KIF2A_HUMAN antibody
      • Kinesin 2 antibody
      • Kinesin heavy chain 2 antibody
      • Kinesin heavy chain member 2 antibody
      • Kinesin heavy chain member 2A antibody
      • Kinesin like protein KIF2A antibody
      • Kinesin-2 antibody
      • Kinesin-like protein KIF2A antibody
      • KNS2 antibody
      • M Kinesin antibody
      see all

    Anti-Kif2a antibody images

    • Kif2a antibody (ab55383) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human testis.
    • Western blot against tagged recombinant protein immunogen using ab55383 Kif2a antibody at 1ug/ml. Predicted band size of immunogen is 101 kDa

    References for Anti-Kif2a antibody (ab55383)

    ab55383 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Xenopus laevis Cell (Spindles assembled in X. laevis cytoplasmic egg ex)
    Specification Spindles assembled in X. laevis cytoplasmic egg ex
    Fixative Formaldehyde and Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Nov 17 2011

    Application Western blot
    Sample Xenopus tropicalis Cell lysate - other (Xenopus laevis and tropicalis cytoplasmic egg extr)
    Loading amount 40 µg
    Specification Xenopus laevis and tropicalis cytoplasmic egg extr
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 17 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"