Overview

  • Product nameAnti-Kif2a antibody
    See all Kif2a primary antibodies
  • Description
    Rabbit polyclonal to Kif2a
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Kif2a.

    (Peptide available as ab129223.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; NIH3T3; MEF1; F9.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 80 kDa).

Target

  • FunctionPlus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
  • Sequence similaritiesBelongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDCBM3 antibody
    • HK2 antibody
    • KIF2 antibody
    • KIF2A antibody
    • KIF2A_HUMAN antibody
    • Kinesin 2 antibody
    • Kinesin heavy chain 2 antibody
    • Kinesin heavy chain member 2 antibody
    • Kinesin heavy chain member 2A antibody
    • Kinesin like protein KIF2A antibody
    • Kinesin-2 antibody
    • Kinesin-like protein KIF2A antibody
    • KNS2 antibody
    • M Kinesin antibody
    see all

Anti-Kif2a antibody images

  • All lanes : Anti-Kif2a antibody (ab86655) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 6 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 7 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 10 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 11 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 12 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 13 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 14 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-Kif2a antibody (ab86655)

ab86655 has not yet been referenced specifically in any publications.

Product Wall

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