The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceKIF2B belongs to the kinesin-like protein family. It is a plus end-directed microtubule-dependent motor required for spindle assembly and chromosome movement. It also has microtubule depolymerization activity.
Cellular localizationCytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; spindle. Kinetochore. Note: Association with kinetochore is transient.
Anti-KIF2B antibody (ab98214) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal thymus lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 76 kDa
References for Anti-KIF2B antibody (ab98214)
This product has been referenced in:
Wang H et al. Expression of dynein, cytoplasmic 2, heavy chain 1 (DHC2) associated with glioblastoma cell resistance to temozolomide. Sci Rep6:28948 (2016).
Read more (PubMed: 27375225) »
Pillai S et al.
Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.
Nat Commun6:10072 (2015).
Read more (PubMed: 26656453) »