Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF3A
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.

  • Positive control
    • WB: HeLa and MDBK cell lysate. ICC/IF: Primary hippocampal neurons. IHC-P: Rat brain tiisue; mouse brain tissue.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100.

Target

  • Function
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIF 3 antibody
    • KIF 3A antibody
    • KIF3 antibody
    • Kif3a antibody
    • KIF3A_HUMAN antibody
    • Kifl antibody
    • Kinesin family member 3A antibody
    • Kinesin family protein 3 antibody
    • Kinesin family protein 3A antibody
    • Kinesin like protein KIF 3A antibody
    • Kinesin like protein KIF3A antibody
    • Kinesin-like protein KIF3A antibody
    • Kns3 antibody
    • Microtubule plus end directed kinesin motor 3A antibody
    • Microtubule plus end-directed kinesin motor 3A antibody
    see all

Images



  • Predicted band size : 85 kDa

    This image is courtesy of an anonymous Abreview

    ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.

    See Abreview

  • Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Hoang-Minh LB  et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). WB ; Human . Read more (PubMed: 27661404) »
  • Schueler M  et al. DCDC2 mutations cause a renal-hepatic ciliopathy by disrupting Wnt signaling. Am J Hum Genet 96:81-92 (2015). IF . Read more (PubMed: 25557784) »

See all 18 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
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Submitted Nov 02 2015

Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Loading amount
100 µg
Specification
MDCK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 14 2013

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount
100 µg
Specification
COS cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (MDBK cells)
Loading amount
100 µg
Specification
MDBK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 13 2013

Application
Western blot
Sample
Syrian Hamster Cell lysate - whole cell (BHK cells)
Loading amount
100 µg
Specification
BHK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Mar 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH-3T3 cells)
Loading amount
100 µg
Specification
NIH-3T3 cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Mar 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (NRK cells)
Loading amount
50 µg
Specification
NRK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Mar 28 2013

Application
Immunocytochemistry
Sample
Mouse Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
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Submitted Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (brain lysate differentially centrifuged, P3)
Loading amount
5 µg
Specification
brain lysate differentially centrifuged, P3
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 24 2011

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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