Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 20421870
FunctionMicrotubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
Sequence similaritiesBelongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.
Western blot - Anti-KIF3A antibody (ab11259)This image is courtesy of an anonymous Abreview
Predicted band size : 85 kDa
This image is courtesy of an anonymous Abreview
ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.
Immunocytochemistry/ Immunofluorescence - Anti-KIF3A antibody (ab11259)Image courtesy of an anonymous Abreview.
ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.
Hoang-Minh LB et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol9:392-402 (2016).
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Smith GA et al. Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease. Hum Mol GenetN/A:N/A (2014).
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