KIF3A antibody (ab11259)

Product Name 

KIF3A antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to KIF3A

Immunogen 

Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IF: 1/100, this concentration is determined by indirect immunofluorescence using frozen sections of rat cerebellum.
WB: 1/2000, this concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. Predicted molecular weight: 85 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat brain homogenate, PC12 cell line.

Cellular localization 

Microtubule

Research areas 

Stem Cells >> Signaling Pathways >> Wnt >> Surface Molecules
Neuroscience >> Neurotransmitter >> Transporters >> Other Transporters
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Adapters
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin

Relevance 

The kinesin superfamily (KIFs) of proteins consists of a class of microtubule-dependent motors that plays a major role in many cellular and developmental functions, including organelle transport, mitosis, meiosis, and possibly long-range signaling in neurons. The KIF proteins are primarily associated with anterograde transport of vesicles and organelles in neurons, epithelial cells, and melanosomes with bidirectional transport of mitochondria and mediating transport between the endoplasmic reticulum (ER) and the Golgi complex. KIF3 motor complex is composed of the KIF3A, KIF3B, and KIF3C motor subunits, that associate as heterodimers, in which KIF3B and KIF3C are alternative partners of KIF3A. KIF3A/B has been shown to function in vivo as a motor for the anterograde fast axonal transport of membranebound organelles in neurons. KIF3 plays a crucial role during development. KIF3A-/- and KIF3B-/- mice do not survive beyond midgestation.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Form 

Liquid

Concentration 

3.600 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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KIF3A antibody images:

References for KIF3A antibody (ab11259)

  This product has been used in: (two most recent references)

Azevedo C et al. Inositol pyrophosphate mediated pyrophosphorylation of AP3B1 regulates HIV-1 Gag release. Proc Natl Acad Sci U S A 106:21161-6 (2009). WB; Human, Mouse. PubMed: 19934039

Gu C et al. The microtubule plus-end tracking protein EB1 is required for Kv1 voltage-gated K+ channel axonal targeting. Neuron 52:803-16 (2006). PubMed: 17145502
If you publish research using ab11259 please let us know so that we can cite the reference on this datasheet.

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