Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF3A
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.

  • Positive control
    • Rat brain homogenate, PC12 cell line.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 85 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 20421870

Target

  • Function
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIF 3 antibody
    • KIF 3A antibody
    • KIF3 antibody
    • Kif3a antibody
    • KIF3A_HUMAN antibody
    • Kifl antibody
    • Kinesin family member 3A antibody
    • Kinesin family protein 3 antibody
    • Kinesin family protein 3A antibody
    • Kinesin like protein KIF 3A antibody
    • Kinesin like protein KIF3A antibody
    • Kinesin-like protein KIF3A antibody
    • Kns3 antibody
    • Microtubule plus end directed kinesin motor 3A antibody
    • Microtubule plus end-directed kinesin motor 3A antibody
    see all

Images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview



  • Predicted band size : 85 kDa

    This image is courtesy of an anonymous Abreview

    ab11259 (1/2000) detecting KIF3A in Human Hela Cells. As a control, cells were transfected with scramble siRNA (left) and KIF3A siRNA (right). Please refer to abreview for further experimental details.

    See Abreview

  • ab11259 staining KIF3A in murine primary hippocampal neurons by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with methanol, blocked with 1% BSA for 1 hour at 22°C and then incubated with ab11259 at a 1/50 dilution for 2 hours at 22°C. The secondary used was a Cy3-conjugated donkey anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Hoang-Minh LB  et al. PCM1 Depletion Inhibits Glioblastoma Cell Ciliogenesis and Increases Cell Death and Sensitivity to Temozolomide. Transl Oncol 9:392-402 (2016). WB ; Human . Read more (PubMed: 27661404) »
  • Schueler M  et al. DCDC2 mutations cause a renal-hepatic ciliopathy by disrupting Wnt signaling. Am J Hum Genet 96:81-92 (2015). IF . Read more (PubMed: 25557784) »

See all 18 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. ab11259 was verified to work by immunohistochemistry using frozen sections of rat cerebellum at a 1:200 dilution. You may find it useful to refer to the paper below that characterises its application. Kinesin and...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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