Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF3A
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 652 and 702 of human KIF3A using the numbering given in entry AAC72294.1

  • Positive control
    • HeLa, 293T or mouse NIH3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIF 3 antibody
    • KIF 3A antibody
    • KIF3 antibody
    • Kif3a antibody
    • KIF3A_HUMAN antibody
    • Kifl antibody
    • Kinesin family member 3A antibody
    • Kinesin family protein 3 antibody
    • Kinesin family protein 3A antibody
    • Kinesin like protein KIF 3A antibody
    • Kinesin like protein KIF3A antibody
    • Kinesin-like protein KIF3A antibody
    • Kns3 antibody
    • Microtubule plus end directed kinesin motor 3A antibody
    • Microtubule plus end-directed kinesin motor 3A antibody
    see all

Images

  • All lanes : Anti-KIF3A antibody (ab95885) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 80 kDa


    Exposure time : 3 minutes
  • Detection of Human KIF3A by Immunoprecipitation, using ab95885 at 3µg/mg lysate. Image shows immunoprecipitated KIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, an antibody recognising a upstream eptiope in lane 1, ab95885 at 1 µg/ml in lane 2, and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab95885 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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