Overview

  • Product name
  • Description
    Mouse polyclonal to KIF3C
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to amino acids 1-793 of Human KIF3C (NP_002245.4).

  • Positive control
    • HeLa cell lysate and KIF3C transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Microtubule-based anterograde translocator for membranous organelles.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin II subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA4058 antibody
    • KIF3C antibody
    • KIF3C variant protein antibody
    • KIF3C_HUMAN antibody
    • Kinesin family member 3C antibody
    • Kinesin like protein KIF3C antibody
    • Kinesin-like protein KIF3C antibody
    • mKIAA4058 antibody
    see all

Anti-KIF3C antibody images

  • Anti-KIF3C antibody (ab89276) at 1 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-KIF3C antibody (ab89276) at 1 µg/ml

    Lane 1 : KIF3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 89 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-KIF3C antibody (ab89276)

ab89276 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
Yes - 0.3% Triton X-100, 30min, R.T.
Fixative
Paraformaldehyde
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Submitted Aug 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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