Overview

  • Product nameAnti-KIF4A antibody
    See all KIF4A primary antibodies
  • Description
    Rabbit polyclonal to KIF4A
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SDETVACMAA AIDTAVEQEA QVETSPETSR SSDAFTTQHA LRQAQMSKEL VELNKALALK EALARKMTQN DSQLQPIQYQ YQDN, corresponding to amino acids of Human KIF4A.

  • Positive control
    • Human bone marrow tissue; A431 cells; RT 4 and U 251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionMotor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • Tissue specificityHighly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • Sequence similaritiesBelongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromokinesin antibody
    • Chromokinesin-A antibody
    • Chromosome associated kinesin KIF4A antibody
    • Chromosome-associated kinesin KIF4A antibody
    • FLJ12530 antibody
    • FLJ12655 antibody
    • FLJ14204 antibody
    • FLJ20631 antibody
    • HSA271784 antibody
    • KIF4 antibody
    • KIF4 G1 antibody
    • KIF4A antibody
    • KIF4A_HUMAN antibody
    • KIF4G1 antibody
    • Kinesin family member 4A antibody
    see all

Anti-KIF4A antibody images

  • ab122228, at 1/50 dilution, staining KIF4A in paraffin-embedded Human bone marrow tissue by Immunohistochemistry.
  • ab122228, at 4 µg/mL, staining KIF4A in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
  • All lanes : Anti-KIF4A antibody (ab122228) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • Immunohistochemical staining of Human bone marrow shows strong nuclear positivity in hematopoietic cells using ab122228

References for Anti-KIF4A antibody (ab122228)

ab122228 has not yet been referenced specifically in any publications.

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