Overview

  • Product name
  • Description
    Goat polyclonal to KIF4A
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide:

    SFFSGCSPIEEEAH

    , corresponding to C terminal amino acids 1219-1232 of Human KIF4A.

Properties

Applications

Our Abpromise guarantee covers the use of ab3815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/100. Can be blocked with Human KIF4A peptide (ab22955).
ICC/IF 1/100.

Target

  • Function
    Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • Tissue specificity
    Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromokinesin antibody
    • Chromokinesin-A antibody
    • Chromosome associated kinesin KIF4A antibody
    • Chromosome-associated kinesin KIF4A antibody
    • FLJ12530 antibody
    • FLJ12655 antibody
    • FLJ14204 antibody
    • FLJ20631 antibody
    • HSA271784 antibody
    • KIF4 antibody
    • KIF4 G1 antibody
    • KIF4A antibody
    • KIF4A_HUMAN antibody
    • KIF4G1 antibody
    • Kinesin family member 4A antibody
    see all

Images

  • ab3815 (1/100) stainning KIF4A in HeLa Cells (blue). Cells were fixed with Methanol and BSA was used as blocking agent. Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-KIF4A antibody (ab3815) at 1/100 dilution

    Lane 1 : Whole cell lysate prepared from HeLa cells synchronized with Nocodazole for 12 hours.
    Lane 2 : As Lane one but with KIF4A siRNA treatment as negative control.

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP conjugated anti goat at 1/5000 dilution
    Developed using the ECL technique

    Observed band size : 140 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Image courtesy of an anonymous Abreview.

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line)
Specification
HeLa cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - triton .15
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
10 µg
Specification
HeLa cells
Treatment
500nM nocodazole for 12 hrs
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted May 28 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
10 µg
Specification
HeLa
Treatment
Nocodazole synchronized for 12hrs
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Mar 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Dec 03 2008

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We do experience occasional batch variation. This often means that it may be necessary for our customers to perform an element of optimisation...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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