Overview

  • Product nameAnti-KIF4A antibody
    See all KIF4A primary antibodies
  • Description
    Rabbit polyclonal to KIF4A
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1182 and the C terminus (residue 1232) of human KIF4A (NP_036442.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab72412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMotor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • Tissue specificityHighly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • Sequence similaritiesBelongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chromokinesin antibody
    • Chromokinesin-A antibody
    • Chromosome associated kinesin KIF4A antibody
    • Chromosome-associated kinesin KIF4A antibody
    • FLJ12530 antibody
    • FLJ12655 antibody
    • FLJ14204 antibody
    • FLJ20631 antibody
    • HSA271784 antibody
    • KIF4 antibody
    • KIF4 G1 antibody
    • KIF4A antibody
    • KIF4A_HUMAN antibody
    • KIF4G1 antibody
    • Kinesin family member 4A antibody
    see all

Anti-KIF4A antibody images

  • All lanes : Anti-KIF4A antibody (ab72412) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 140 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of KIF4A by Western Blot of Immunprecipitate.
    ab72412 at 1µg/ml staining KIF4A in HeLa whole cell lysate immunoprecipitated using ab72412 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References for Anti-KIF4A antibody (ab72412)

ab72412 has not yet been referenced specifically in any publications.

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