Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. Contains 1 kinesin motor domain.
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
Detection of Kinesin Heavy Chain in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab187693 at 6 µg/mg lysate. An alternative anti-Kinesin Heavy Chain antibody was used at 1 µg/ml for subsequent western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.