Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF5B
  • Specificity
    This antibody detects total level of endogenous kinesin protein.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Human KIF5B. Stalk domain of human KIF5B (amino acids 331-906) expressed in E. coli (FKHC3).

Properties

Applications

Our Abpromise guarantee covers the use of ab25715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000. Predicted molecular weight: 110 kDa.

Target

  • Function
    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • Sequence similarities
    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.
  • Domain
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization
    Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • kinesin 1 (110-120kD) antibody
    • Kinesin 1 heavy chain antibody
    • Kinesin family member 5B antibody
    • Kinesin heavy chain antibody
    • kinesin, heavy chain, ubiquitous antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS 1 antibody
    • KNS antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

Anti-KIF5B antibody images

  • All lanes : Anti-KIF5B antibody (ab25715)

    Lane 1 : Human HEK cell line
    Lane 2 : Porcine brain


    Predicted band size : 110 kDa
  • ab25715 staining KIF5B in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton and blocked with 0.1% BSA for 16 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS + 0.1% BSA) for 1 hour at 37°C. An Alexa Fluor®546-conjugated donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-KIF5B antibody (ab25715)

This product has been referenced in:
  • Malikov V  et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB, ICC/IF . Read more (PubMed: 25818806) »
  • Chu Y  et al. Alterations in axonal transport motor proteins in sporadic and experimental Parkinson's disease. Brain 135:2058-73 (2012). ICC/IF ; Rat . Read more (PubMed: 22719003) »

See all 2 Publications for this product

Product Wall

The immunogen was FKHC3 stalk coiled-coiled domain (aa331-906) of human neuronal kinesin heavy chain. This pAb detects total level of endogeneous kinesin protein. However, we have not and we do not know of any study that has examined its reactivity wit...

Read More

Thank you for contacting us. The immunogen sequence is as follows: VNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLE AFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKL KTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELA...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Cells)
Loading amount
250 µg
Specification
A549 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up