Overview

  • Product nameAnti-KIF5B antibody
    See all KIF5B primary antibodies
  • Description
    Rabbit polyclonal to KIF5B
  • SpecificityThis antibody is specific for KIF5B and does not detect other kinesin isotypes.
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human KIF5B aa 376-396.
    Sequence:

    IDEQFDKEKANLEAFTVDKDI


    Database link: P33176
    (Peptide available as ab41782)

  • Positive control
    • Mouse neuronal cell extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human KIF5B peptide (ab41782).
IP Use at an assay dependent concentration.

Target

  • FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.
  • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • kinesin 1 (110-120kD) antibody
    • Kinesin 1 heavy chain antibody
    • Kinesin family member 5B antibody
    • Kinesin heavy chain antibody
    • kinesin, heavy chain, ubiquitous antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS 1 antibody
    • KNS antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

Anti-KIF5B antibody images

  • ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.



  • Predicted band size : 110 kDa

    Shows ab5629 used for a Western blot of Mouse neuronal cell extract.

  • ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody.

    This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.

    See Abreview

References for Anti-KIF5B antibody (ab5629)

This product has been referenced in:
  • Brown AK  et al. Opposing microtubule motors control motility, morphology and cargo segregation during ER-to-Golgi transport. Biol Open 3:307-13 (2014). Read more (PubMed: 24705013) »
  • Barry J  et al. Activation of conventional kinesin motors in clusters by Shaw voltage-gated K+ channels. J Cell Sci 126:2027-41 (2013). Read more (PubMed: 23487040) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (forebrain)
Total protein in input 20000 µg
Specification forebrain
Immuno-precipitation step Protein A
Username

Dr. Kieran Brickley

Verified customer

Submitted May 01 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (oligodendrocyte cell line Olineu)
Specification oligodendrocyte cell line Olineu
Fixative Paraformaldehyde
Blocking step Other as blocking agent for 30 minute(s) · Concentration: 6%
Username

Dr. katarina trajkovic

Verified customer

Submitted Feb 22 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"