The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human KIF5B peptide (ab41782).
Use at an assay dependent concentration.
FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. Contains 1 kinesin motor domain.
DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37ºC were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1:2000). Images were taken on wide-field fluorescent microscope at 63X magnification.
Western blot - Anti-KIF5B antibody (ab5629)
Predicted band size : 110 kDa
Shows ab5629 used for a Western blot of Mouse neuronal cell extract.
ab5629 at 1/200 dilution staining the mouse oligodendrocyte cell line, Olineu, by immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated secondary antibody.
This image is courtesy of an Abreview submitted by Katarina Trajko on 22 February 2006.