Overview

  • Product name
    Anti-KIF5B antibody [H2]
    See all KIF5B primary antibodies
  • Description
    Mouse monoclonal [H2] to KIF5B
  • Specificity
    ab23664 recognises the heavy chain of kinesin.
  • Tested applications
    Suitable for: Electron Microscopy, IHC-FoFr, ICC, WB, IP, RIAmore details
  • Species reactivity
    Reacts with: Rat, Chicken, Sea urchin
    Predicted to work with: Mammal
  • Immunogen

    Full length protein corresponding to Cow KIF5B.

  • Epitope
    The epitope recognised by ab23664 is located near the N terminus of Kinesin heavy chain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    H2
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
IHC-FoFr
ICC
WB
IP
RIA
  • Application notes
    Electron Microscopy: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IHC-F: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 109 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
    • Sequence similarities
      Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
      Contains 1 kinesin motor domain.
    • Domain
      Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
    • Cellular localization
      Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    References for Anti-KIF5B antibody [H2] (ab23664)

    This product has been referenced in:
    • Astanina K & Jacob R KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Cell Mol Life Sci 67:1331-42 (2010). IP . Read more (PubMed: 20094756) »
    • Zhang L  et al. {gamma}-Diketone Axonopathy: Analyses of Cytoskeletal Motors and Highways in CNS Myelinated Axons. Toxicol Sci 117:180-9 (2010). WB ; Rat . Read more (PubMed: 20554699) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting Abcam regarding ab23664.

    I have run the BLAST search using the immunogen sequence for this antibody. I am only pulling up 100% with the heavy chain of kinesin-1. None of the other kinesins come up. I would predict th...

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    Thank you for your enquiry. The immunogen for this antibody was kinesin isolated from bovine brain. The antibody is described in detail in one of the references listed towards the bottom of the datasheet: Pfister KK, Wagner MC, Stenoien DL, Bra...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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