SpecificityThe antibody reacts with heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule.
The antibody does not react with kinesin bound to taxol-stabilized microtubules.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. Contains 1 kinesin motor domain.
DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.