Anti-KIF5B antibody [KN-03] (ab11883)

Overview

  • Product nameAnti-KIF5B antibody [KN-03]
    See all KIF5B primary antibodies
  • Description
    Mouse monoclonal [KN-03] to KIF5B
  • SpecificityThe antibody reacts with heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. The antibody recognizes kinesin bound to taxol-stabilized microtubules.
  • Tested applicationsSuitable for: ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Enriched fraction of porcine brain kinesin.

  • EpitopeEpitope mapping has revealed that antibodies ab11882 and ab11883 react with different sets of fragments.

Properties

Applications

Our Abpromise guarantee covers the use of ab11883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ICC
  • Application notesICC: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
    • Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
      Contains 1 kinesin motor domain.
    • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
    • Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
    • Information by UniProt
    • Database links
    • Alternative names
      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    Anti-KIF5B antibody [KN-03] images

    • ab11883 staining rat neuronal stem cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Saponin, prior to blocking with 10% serum for 1 hour at 21°C.  The primary antibody was diluted 1/500 with PBS + 1% FCS and incubated with the sample for 18 hours at 4°C.  A Cy5® conjugated goat anti-mouse IgG antibody was used as the secondary.

      See Abreview

    References for Anti-KIF5B antibody [KN-03] (ab11883)

    ab11883 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (neuronale stem cells)
    Specification neuronale stem cells
    Fixative Paraformaldehyde
    Permeabilization Yes - 0,5% Saponin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Dominik Schelshorn

    Verified customer

    Submitted Feb 01 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"