SpecificityThe antibody reacts with heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule.
The antibody recognizes kinesin bound to taxol-stabilized microtubules.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily. Contains 1 kinesin motor domain.
DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody [KN-03] (ab11883)This image is courtesy of an Abreview submitted by Dr Dominik Schelshorn
ab11883 staining rat neuronal stem cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Saponin, prior to blocking with 10% serum for 1 hour at 21°C. The primary antibody was diluted 1/500 with PBS + 1% FCS and incubated with the sample for 18 hours at 4°C. A Cy5® conjugated goat anti-mouse IgG antibody was used as the secondary.