Overview

  • Product nameAnti-KIF5B antibody [SUK-4]
    See all KIF5B primary antibodies
  • Description
    Mouse monoclonal [SUK-4] to KIF5B
  • SpecificityThis antibody recognizes an epitope at the C terminus of the motor domain.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human, Drosophila melanogaster, Sea Urchin
  • Immunogen

    Full length native protein (purified) corresponding to KIF5B.

  • General notes


    This antibody inhibits kinesin induced microtubule translocation in in vitro motility assays. Kinesin heavy chain localizes to sites of vesicular trafficking, especially microtubule pathways in neuronal cells and the Golgi in cultured non neuronal cell types.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.03% Thimerosal (merthiolate).
    Constituents: PBS.
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody inhibits kinesin induced microtubule translocation in in vitro motility assays. Kinesin heavy chain localizes to sites of vesicular trafficking, especially microtubule pathways in neuronal cells and the Golgi in cultured non neuronal cell types.
  • ClonalityMonoclonal
  • Clone numberSUK-4
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 120 kDa.
ICC/IF 1/500 - 1/1000. PubMed: 19743964

Target

  • FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.
  • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • kinesin 1 (110-120kD) antibody
    • Kinesin 1 heavy chain antibody
    • Kinesin family member 5B antibody
    • Kinesin heavy chain antibody
    • kinesin, heavy chain, ubiquitous antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS 1 antibody
    • KNS antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

Anti-KIF5B antibody [SUK-4] images

  • Ab28060, at a dilution of 1/100, staining KIF5B in MeOH fixed HeLa cells by Immunofluorescence.

References for Anti-KIF5B antibody [SUK-4] (ab28060)

This product has been referenced in:
  • Kathleen Baxter K  et al. The neurogenic basic helix-loop-helix transcription factor NeuroD6 concomitantly increases mitochondrial mass and regulates cytoskeletal organization in the early stages of neuronal differentiation. ASN Neuro 1: (2009). WB, ICC/IF ; Rat . Read more (PubMed: 19743964) »

See 1 Publication for this product

Product Wall

Thank you for your reply.


I am glad there is another construct available for you to work with. I wish you the best of luck with your experiments!


Please do not hesitate to contact me if I can be of any further assistance.

Thank you for contacting Abcam regarding ab28060.


Unfortunately we have not mapped the specific epitope for this monoclonal antibody and thus we do not know which part of the protein is specifically recognized by it. The antibody was ra...

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