• Product nameAnti-KIF7 antibody
  • Description
    Rabbit polyclonal to KIF7
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide designed within residues: KRSLCSEGRQ APGNEDELHL APELLWLSPL TEGAPRTREE TRDLVHAPLP L, corresponding to amino acids 1225-1275 of Human KIF7 (NP_940927.2)

  • Positive control
    • HeLa or 293T cell lysate
  • General notesShelf Life: 1 year from date of receipt



Our Abpromise guarantee covers the use of ab95884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 151 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionActs as both a negative and positive regulator of sonic hedgehog (Shh) pathway, acting downstream of SMO. Negatively regulates the pathway by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates the pathway by preventing the processing of the transcription factor GLI3 into its repressor form. Required for efficient localization of GLI3 to cilia in response to Shh. May also act as a ciliary motor.
    • Tissue specificityEmbryonic stem cells, melanotic melanoma and Jurkat T-cells.
    • Sequence similaritiesBelongs to the kinesin-like protein family. KIF27 subfamily.
      Contains 1 kinesin-motor domain.
    • Cellular localizationCell projection > cilium. SMO is required for its accumulation within cilia. Moves from the cilia base to the cilia tip in response to activation of the Shh pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • EQYK340 antibody
      • kif7 antibody
      • KIF7_HUMAN antibody
      • kinesin family member 7 antibody
      • kinesin like protein KIF7 antibody
      • Kinesin-like protein kif7 antibody
      • UNQ340 antibody
      see all

    Anti-KIF7 antibody images

    • All lanes : Anti-KIF7 antibody (ab95884) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 151 kDa

      Exposure time : 30 seconds

    References for Anti-KIF7 antibody (ab95884)

    This product has been referenced in:
    • Ho J  et al. Downregulation of the gli transcription factors regulator Kif7 facilitates cell survival and migration of choriocarcinoma cells. PLoS One 9:e108248 (2014). WB ; Human . Read more (PubMed: 25265279) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Mouse Cell lysate - whole cell (MEF)
    Specification MEF
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Mar 03 2014