Recombinant Anti-KIFAP3 antibody [EPR5599] (ab109026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5599] to KIFAP3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KIFAP3 antibody [EPR5599]
See all KIFAP3 primary antibodies -
Description
Rabbit monoclonal [EPR5599] to KIFAP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, SH-SY5Y, C6, 293T, and Human fetal kidney lysates, Human bladder carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5599 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex. -
Sequence similarities
Contains 5 ARM repeats. -
Post-translational
modificationsPhosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1. - Information by UniProt
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Database links
- Entrez Gene: 22920 Human
- Entrez Gene: 16579 Mouse
- Entrez Gene: 289168 Rat
- Omim: 601836 Human
- SwissProt: Q92845 Human
- SwissProt: P70188 Mouse
- SwissProt: D3ZK15 Rat
- SwissProt: D3ZWA5 Rat
see all -
Alternative names
- FLJ22818 antibody
- KAP 3 antibody
- KAP-3 antibody
see all
Images
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All lanes : Anti-KIFAP3 antibody [EPR5599] (ab109026) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : C6 lysate
Lane 4 : 293T lysate
Lane 5 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 91 kDa -
Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue using ab109026 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109026 has not yet been referenced specifically in any publications.