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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BW19
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab172620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 74 kDa.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab172620 observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 at 1/10 dilution.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 at 1/50 dilution.
Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 at 1/50 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"