Overview

  • Product name
    Anti-KIFC1 antibody [11445]
    See all KIFC1 primary antibodies
  • Description
    Rabbit monoclonal [11445] to KIFC1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BW19

  • Positive control
    • Human tonsil tissue; Human cervical carcinoma; HeLa, HepG2 and 293T cell lysates; Immunoprecipitation pellet from 293T cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
    • Sequence similarities
      Belongs to the kinesin-like protein family. NCD subfamily.
      Contains 1 kinesin-motor domain.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • HSET antibody
      • HSET KNSL2 antibody
      • KIF C1 antibody
      • KIFC 1 antibody
      • KIFC1 antibody
      • KIFC1_HUMAN antibody
      • Kinesin family member C1 antibody
      • Kinesin like 2 antibody
      • Kinesin like protein 2 antibody
      • Kinesin like protein KIFC1 antibody
      • Kinesin related protein HSET antibody
      • Kinesin-like protein 2 antibody
      • Kinesin-like protein KIFC1 antibody
      • Kinesin-related protein HSET antibody
      • KNSL 2 antibody
      • KNSL2 antibody
      • MGC1202 antibody
      • MGC149736 antibody
      • MGC149737 antibody
      • Mitotic kinesin like protein 2 antibody
      • MKLP 2 antibody
      • MKLP2 antibody
      • RAB6 KIFL antibody
      • Rabkinesin 6 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab172620 observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-KIFC1 antibody [11445] (ab172620) at 1/10000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : 293T lysate
      Lane 3 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 74 kDa

    • Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 at 1/10 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 at 1/50 dilution.

    • Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 at 1/50 dilution.

    References

    This product has been referenced in:
    • She ZY  et al. Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. Oncotarget 8:36469-36483 (2017). Read more (PubMed: 28430595) »
    • Xiao YX  et al. C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma. Oncotarget 8:61373-61384 (2017). WB ; Human . Read more (PubMed: 28977870) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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