Overview

  • Product nameAnti-KIFC1 antibody
    See all KIFC1 primary antibodies
  • Description
    Mouse monoclonal to KIFC1
  • Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
  • Immunogen

    Recombinant fragment: MDPQRSPLLE VKGNIELKRP LIKAPSQLPL SGSRLKRRPD QMEDGLEPEK KRTRGLGATT KITTSHPRVP SLTTVPQTQG QTTAQKVSKK TGPRCSTAIA , corresponding to amino acids 53-152 of Human KIFC1

Properties

Applications

Our Abpromise guarantee covers the use of ab55388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMinus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
  • Sequence similaritiesBelongs to the kinesin-like protein family. NCD subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSET antibody
    • HSET KNSL2 antibody
    • KIF C1 antibody
    • KIFC 1 antibody
    • KIFC1 antibody
    • KIFC1_HUMAN antibody
    • Kinesin family member C1 antibody
    • Kinesin like 2 antibody
    • Kinesin like protein 2 antibody
    • Kinesin like protein KIFC1 antibody
    • Kinesin related protein HSET antibody
    • Kinesin-like protein 2 antibody
    • Kinesin-like protein KIFC1 antibody
    • Kinesin-related protein HSET antibody
    • KNSL 2 antibody
    • KNSL2 antibody
    • MGC1202 antibody
    • MGC149736 antibody
    • MGC149737 antibody
    • Mitotic kinesin like protein 2 antibody
    • MKLP 2 antibody
    • MKLP2 antibody
    • RAB6 KIFL antibody
    • Rabkinesin 6 antibody
    see all

Anti-KIFC1 antibody images

  • KIFC1 antibody (ab55388) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
  • Western blot against tagged recombinant protein immunogen using ab55388 KIFC1 antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa
  • ab55388 at 10 ug/ml staining KIFC1 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55388 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55388, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Anti-KIFC1 antibody (ab55388)

ab55388 has not yet been referenced specifically in any publications.

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I am sorry for the confusion caused by the western blot presented on the datasheet of ab55388. The band observed in this Western blot was indeed ˜34 kDa. However, the sample lo...

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