Anti-KIN antibody [2490D1a] (ab67100)

Overview

• Product name
  Anti-KIN antibody [2490D1a]
  See all KIN primary antibodies
• Description
  Mouse monoclonal [2490D1a] to KIN
• Tested applications
  Suitable for: WB, Dot blotmore details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment (Human) from an internal sequence of KIN.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: 0.05% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and was filtered through a 0.22µm membrane.
• Clonality
  Monoclonal
• Clone number
  2490D1a
• Isotype
  IgG1
• Research areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • DNA Damage Recognition

Associated products

• Compatible Secondaries
  • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Target

• Function
  Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.
• Tissue specificity
  Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).
• Sequence similarities
  Belongs to the KIN17 family.
  Contains 1 C2H2-type zinc finger.
• Domain
  The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.
• Cellular localization
  Nucleus. Cytoplasm. During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.

• Target information above from: UniProt accession O60870 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 22944 Human
  • Omim: 601720 Human
  • SwissProt: O60870 Human
  • Unigene: 397918 Human
• Alternative names
  • antigenic determinant of recA protein homolog antibody
  • Binding to curved DNA antibody
  • BTCD antibody

  • DNA/RNA-binding protein KIN17 antibody
  • HsKin17 protein antibody
  • KIN antibody
  • KIN antigenic determinant of recA protein homolog mouse antibody
  • Kin17 protein antibody
  • KIN17_HUMAN antibody

  see all

Protocols

• Western blot protocols