Overview

  • Product name
    Anti-KIN antibody [2490D1a]
    See all KIN primary antibodies
  • Description
    Mouse monoclonal [2490D1a] to KIN
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human) from an internal sequence of KIN.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and was filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2490D1a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight of full length protein: 45 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.
    • Tissue specificity
      Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).
    • Sequence similarities
      Belongs to the KIN17 family.
      Contains 1 C2H2-type zinc finger.
    • Domain
      The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.
    • Cellular localization
      Nucleus. Cytoplasm. During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigenic determinant of recA protein homolog antibody
      • Binding to curved DNA antibody
      • BTCD antibody
      • DNA/RNA-binding protein KIN17 antibody
      • HsKin17 protein antibody
      • KIN antibody
      • KIN antigenic determinant of recA protein homolog mouse antibody
      • Kin17 protein antibody
      • KIN17_HUMAN antibody
      see all

    Images

    References

    ab67100 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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