Overview

  • Product name
  • Description
    Rabbit polyclonal to KIN
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from a region between residues 208-262 of human KIN

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purification notes
    Purified by peptide affinity chromatography. Antibody is lyophilized from PBS buffer with 2% sucrose.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.
  • Tissue specificity
    Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).
  • Sequence similarities
    Belongs to the KIN17 family.
    Contains 1 C2H2-type zinc finger.
  • Domain
    The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.
  • Cellular localization
    Nucleus. Cytoplasm. During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigenic determinant of recA protein homolog antibody
    • Binding to curved DNA antibody
    • BTCD antibody
    • DNA/RNA-binding protein KIN17 antibody
    • HsKin17 protein antibody
    • KIN antibody
    • KIN antigenic determinant of recA protein homolog mouse antibody
    • Kin17 protein antibody
    • KIN17_HUMAN antibody
    see all

Images

  • Anti-KIN antibody (ab22842) at 1 µg/ml + HepG2 cell lysate with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,025.

    Performed under reducing conditions.

    Predicted band size : 45 kDa
  • Paraffin embeded tissue: Human kidney, Cells with positive label (indicated with arrows): Epithelial cells of renal tubule, Antibody concentration: 4-8 µg/ml, Magnification: x400
  • Paraffin embeded tissue: Human spermatophore, Cells with positive label (indicated with arrows): Epithelial cells, Antibody concentration: 4-8 µg/ml, Magnification: x400

References

ab22842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab22842.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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