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Rabbit polyclonal to Kindlin 2
Predicted to work with:
Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
Synthetic peptide within Human Kindlin 2 aa 175-225. The exact sequence is proprietary. (NP_006823.1)
HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 78 kDa.
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
Ubiquitous. Found in numerous tumor tissues.
Belongs to the kindlin family.
Contains 1 FERM domain. Contains 1 PH domain.
The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
Information by UniProt
Western blot - Anti-Kindlin 2 antibody (ab194967)
All lanes :
Anti-Kindlin 2 antibody (ab194967) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lane 4 :
TCMK-1 whole cell lysate
Lane 5 :
NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size :
Exposure time :
Cell lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-Kindlin 2 antibody (ab194967)
Detection of Kindlin 2 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194967 at 6 µg/mg lysate for IP and at 0.1 µg/mL for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"