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Overview
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Product nameAnti-Kindlin 2 antibody
See all Kindlin 2 primary antibodies -
DescriptionRabbit polyclonal to Kindlin 2
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Host speciesRabbit
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Tested applicationsSuitable for: IP, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human Kindlin 2 aa 175-225. The exact sequence is proprietary. (NP_006823.1)
Sequence:SGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVS Q
Database link: Q96AC1 -
Positive control
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab194967 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 78 kDa. |
Target
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FunctionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
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Tissue specificityUbiquitous. Found in numerous tumor tissues.
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Sequence similaritiesBelongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain. -
DomainThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
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Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
- Information by UniProt
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Database links
- Entrez Gene: 452910 Chimpanzee
- Entrez Gene: 100401538 Common marmoset
- Entrez Gene: 616981 Cow
- Entrez Gene: 490693 Dog
- Entrez Gene: 101125050 Gorilla
- Entrez Gene: 100049968 Horse
- Entrez Gene: 10979 Human
- Entrez Gene: 218952 Mouse
see all -
Alternative names
- FLJ34213 antibody
- FLJ44462 antibody
- UNC112 antibody
see all
Images
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Western blot - Anti-Kindlin 2 antibody (ab194967)All lanes : Anti-Kindlin 2 antibody (ab194967) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Exposure time: 30 secondsCell lysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-Kindlin 2 antibody (ab194967)Detection of Kindlin 2 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194967 at 6 µg/mg lysate for IP and at 0.1 µg/mL for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
References
ab194967 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"