Anti-Kinesin 5 A + B + C antibody (ab62104)

Overview

  • Product nameAnti-Kinesin 5 A + B + C antibody
    See all Kinesin 5 A + B + C primary antibodies
  • Description
    Rabbit polyclonal to Kinesin 5 A + B + C
  • Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Raised against a 20 amino acid peptide from near the carboxy terminus of human kinesin 5 (GenBank accession no. Q12840).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab62104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/400. (see Abreview)
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceThe kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
  • Cellular localizationUniformly distributed between soma and neurites in hippocampal neurons
  • Database links
  • Alternative names
    • KIF5A antibody
    • KIF5B antibody
    • KIF5C antibody
    • Kinesin family member 5A antibody
    • Kinesin family member 5B antibody
    • Kinesin family member 5C antibody
    • KINH antibody
    • KINN antibody
    • NKHC antibody
    • NKHC2 antibody
    see all

Anti-Kinesin 5 A + B + C antibody images

  • Lane 1 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 0.5 µg/ml
    Lane 2 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 110 kDa
    Observed band size : 116 kDa (why is the actual band size different from the predicted?)
  • ab62104 at 5 µg/ml staining Kinesin 5 A + B + C in human K562 cells by Immunocytochemistry.

  • Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.

References for Anti-Kinesin 5 A + B + C antibody (ab62104)

This product has been referenced in:
  • Su B  et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . Read more (PubMed: 24302729) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Permeabilization Yes - triton 0.3%
Fixative Lana's Fix
Username

Abcam user community

Verified customer

Submitted Jul 16 2013

We publish all the data we receive in Abreviews from customers, in same way they submit to us. Customer have used non reducing conditions however we have tested this antibody under reducing/ denaturing conditions so this antibody is fully guaranteed fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa Cells)
Specification HeLa Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (brain, embryonic E18)
Total protein in input 1000 µg
Specification brain, embryonic E18
Immuno-precipitation step Protein G
Username

Lena

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain Lysate loaded onto a 5-20% sucrose gradient)
Loading amount 10 µg
Specification Brain Lysate loaded onto a 5-20% sucrose gradient
Gel Running Conditions Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"