Anti-Kinesin 5 A + B + C antibody (ab62104)

Overview

  • Product name
    Anti-Kinesin 5 A + B + C antibody
    See all Kinesin 5 A + B + C primary antibodies
  • Description
    Rabbit polyclonal to Kinesin 5 A + B + C
  • Tested applications
    Suitable for: ICC/IF, IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Raised against a 20 amino acid peptide from near the carboxy terminus of human kinesin 5 (GenBank accession no. Q12840).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
IP 1/400. (see Abreview)
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
  • Cellular localization
    Uniformly distributed between soma and neurites in hippocampal neurons
  • Database links
  • Alternative names
    • KIF5A antibody
    • KIF5B antibody
    • KIF5C antibody
    • Kinesin family member 5A antibody
    • Kinesin family member 5B antibody
    • Kinesin family member 5C antibody
    • KINH antibody
    • KINN antibody
    • NKHC antibody
    • NKHC2 antibody
    see all

Images

  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Kinesin 5 A + B + C with Anti-Kinesin 5 A + B + C antibody (ab62104) at 5μg/ml.
  • Lane 1 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 0.5 µg/ml
    Lane 2 : Anti-Kinesin 5 A + B + C antibody (ab62104) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate


    Predicted band size : 110 kDa
    Observed band size : 116 kDa (why is the actual band size different from the predicted?)
  • ab62104 at 5 µg/ml staining Kinesin 5 A + B + C in human K562 cells by Immunocytochemistry.

  • Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.

References

This product has been referenced in:
  • Sherman MA  et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). Read more (PubMed: 27629715) »
  • Su B  et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . Read more (PubMed: 24302729) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Permeabilization
Yes - triton 0.3%
Fixative
Lana's Fix
Username

Abcam user community

Verified customer

Submitted Jul 16 2013

We publish all the data we receive in Abreviews from customers, in same way they submit to us. Customer have used non reducing conditions however we have tested this antibody under reducing/ denaturing conditions so this antibody is fully guaranteed fo...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (brain, embryonic E18)
Total protein in input
1000 µg
Specification
brain, embryonic E18
Immuno-precipitation step
Protein G
Username

Lena

Verified customer

Submitted Sep 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Lysate loaded onto a 5-20% sucrose gradient)
Loading amount
10 µg
Specification
Brain Lysate loaded onto a 5-20% sucrose gradient
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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