Anti-KIF5A antibody (ab118534)
Key features and details
- Rabbit polyclonal to KIF5A
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5A antibody
See all KIF5A primary antibodies -
Description
Rabbit polyclonal to KIF5A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human KIF5A (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: NP_004975.2 -
Positive control
- Human Brain, cortex tissue; K562 cell lysate; K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 117 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 117 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 5 µg/ml. |
Target
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Function
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). -
Tissue specificity
Distributed throughout the CNS but is highly enriched in subsets of neurons. -
Involvement in disease
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3798 Human
- Entrez Gene: 16572 Mouse
- Entrez Gene: 314906 Rat
- Omim: 602821 Human
- SwissProt: Q12840 Human
- SwissProt: P33175 Mouse
- SwissProt: Q6QLM7 Rat
- Unigene: 151219 Human
see all -
Alternative names
- D12S1889 antibody
- KIF 5A antibody
- Kif5a antibody
see all
Images
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Lane 1 : Anti-KIF5A antibody (ab118534) at 0.5 µg/ml
Lane 2 : Anti-KIF5A antibody (ab118534) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 117 kDa -
Human Brain, Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab118534 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunocytochemical analysis of KIF5A expression in K562 cells using ab118534 at 5 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab118534 has been referenced in 2 publications.
- Tucker T et al. Myocardin is Involved in Mesothelial-Mesenchymal Transition of Human Pleural Mesothelial Cells. Am J Respir Cell Mol Biol N/A:N/A (2019). PubMed: 30605348
- Kamata H et al. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis. Sci Rep 7:4556 (2017). PubMed: 28676645