• Product nameAnti-Kinesin 5A antibody
    See all Kinesin 5A primary antibodies
  • Description
    Rabbit polyclonal to Kinesin 5A
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to 20 internal sequence amino acids of Human Kinesin 5A (NP_004975.2).

  • Positive control
    • Human Brain, cortex tissue; K562 cell lysate; K562 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab118534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 117 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 5 µg/ml.


  • FunctionMicrotubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificityDistributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in diseaseDefects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similaritiesBelongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

Anti-Kinesin 5A antibody images

  • Human Brain, Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab118534 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-Kinesin 5A antibody (ab118534) at 0.5 µg/ml
    Lane 2 : Anti-Kinesin 5A antibody (ab118534) at 1 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate

    Predicted band size : 117 kDa
  • Immunocytochemical analysis of Kinesin 5A expression in K562 cells using ab118534 at 5 µg/ml.

References for Anti-Kinesin 5A antibody (ab118534)

ab118534 has not yet been referenced specifically in any publications.

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