• Product nameAnti-Kinesin 5A antibody
    See all Kinesin 5A primary antibodies
  • Description
    Rabbit polyclonal to Kinesin 5A
  • SpecificityThis antibody is specific for kinesin 5A and does not detect other kinesin isotypes.
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Kinesin 5A aa 1007-1027.


    (Peptide available as ab41781)

  • Positive control
    • Mouse neuronal cell extract.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesKinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab5628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 20094756
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).Can be blocked with Kinesin 5A peptide (ab41781).


  • FunctionMicrotubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificityDistributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in diseaseDefects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similaritiesBelongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localizationCytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

Anti-Kinesin 5A antibody images

  • Anti-Kinesin 5A antibody (ab5628) + mouse neuronal cell extract

    HRP-conjugated anti rabbit antibody

    Predicted band size : 117 kDa
  • Anti-Kinesin 5A antibody (ab5628) + human retinal extract

    HRP-conjugated anti-rabbit antibody

    Predicted band size : 117 kDa
  • ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Kinesin 5A antibody (ab5628)

This product has been referenced in:
  • Roche SL  et al. Loss of glial neurofascin155 delays developmental synapse elimination at the neuromuscular junction. J Neurosci 34:12904-18 (2014). Mouse . Read more (PubMed: 25232125) »
  • Cartelli D  et al. Microtubule alterations occur early in experimental parkinsonism and the microtubule stabilizer epothilone D is neuroprotective. Sci Rep 3:1837 (2013). WB ; Mouse . Read more (PubMed: 23670541) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences....

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount 10 µg
Specification Human SH-SY5Y neuroblastoma cells
Gel Running Conditions Reduced Denaturing (7.5 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (Forebrain)
Total protein in input 20000 µg
Specification Forebrain
Immuno-precipitation step Protein A

Dr. Kieran Brickley

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Rat whole brain)
Loading amount 50 µg
Specification Rat whole brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 15 2009