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Our Abpromise guarantee covers the use of ab5630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18334549|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human Kinesin 5C peptide (ab41784).|
Shows a Western blot of kinesin 5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.