Overview

  • Product name
  • Description
    Rabbit polyclonal to Kinesin 5C
  • Specificity
    This antibody is specific for kinesin 5C and does not detect other kinesin isotypes.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Kinesin 5C aa 938-957.
    Sequence:

    AVHAIRGGGGSSSNSTHYQK


    (Peptide available as ab41784)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18334549
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human Kinesin 5C peptide (ab41784).
IP 1/4000.

Target

  • Function
    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • Tissue specificity
    Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDCBM2 antibody
    • FLJ44735 antibody
    • KIAA0531 antibody
    • KIF 5C antibody
    • Kif5c antibody
    • KIF5C protein antibody
    • KIF5C_HUMAN antibody
    • Kinesin family member 5C antibody
    • Kinesin heavy chain isoform 5C antibody
    • Kinesin heavy chain neuron specific 2 antibody
    • Kinesin heavy chain neuron specific antibody
    • Kinesin heavy chain neuron-specific 2 antibody
    • Kinesin5C antibody
    • KINN antibody
    • MGC111478 antibody
    • NKHC 2 antibody
    • NKHC antibody
    • NKHC-2 antibody
    • NKHC2 antibody
    see all

Images



  • Predicted band size : 110 kDa

    Shows a Western blot of kinesin 5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.

  • ab5630 staining Kinesin 5C in COS-7 cells by Immunocytochemistry/ Immunofluorescence.
    GFP-Kif5c behaviour in living cells. Cropped still images from a time-lapse recording of GFP-Kif5c puncta (green) moving along a MT labelled with mCherry–-tubulin (red) in a live COS-7 cell. The GFP-Kif5c puncta move from right to left. Scale bar, 2 µm. The graph shows the respective velocity of the GFP-Kif5c puncta seen over time, demonstrating the rapid changes in velocity of those puncta.
  • IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Poirier K  et al. Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical development and microcephaly. Nat Genet 45:639-47 (2013). Read more (PubMed: 23603762) »
  • Dunn S  et al. Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. J Cell Sci 121:1085-95 (2008). ICC/IF ; Monkey . Read more (PubMed: 18334549) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences....

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount
10 µg
Specification
Human SH-SY5Y neuroblastoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (forebrain)
Specification
forebrain
Immuno-precipitation step
Protein A
Username

Dr. Kieran Brickley

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Recombinant protein (HEK 293 cells)
Loading amount
50 µg
Specification
HEK 293 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
100 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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